Journal: Nano Convergence

Article Title: Efficient methods of isolation and purification of extracellular vesicles

doi: 10.1186/s40580-025-00509-x

Figure Lengend Snippet: Nanoliter scale fluidic controls and microfluidic separation techniques and their conditions for exosome separation. A Inertial separation uses the difference responses and separates them in the concentrating location of the particle according to the difference in inertial forces. B Pressure field flow fractionation (pFFF) that sorts of particles by external force and then separates particles according to different diffusion distances. C Acoustic separation that separates the fluid into a node or anti-node according to the particle characteristics by giving a constant vibration to the fluid. D Pinched flow fractionation (PFF), which sorts particles on the wall and separates them according to the difference in center of gravity. E Biologically intact exosome separation technology (BEST) amplifies the difference in the center of gravity of particles by controlling the flow rate in different fluid geometry. \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{WL}$$\end{document} F WL : wall-induced lift force, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{SL}$$\end{document} F SL : shear gradient lift force, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{P}$$\end{document} F P : force by pressure, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{d}$$\end{document} F d : force by diffusion, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{ac}$$\end{document} F ac : force by an acoustic wave, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{drag}$$\end{document} F drag : drag force, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{sc}$$\end{document} F sc : force by suction flow. \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${Q}_{s}$$\end{document} Q s : sample flow rate, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${Q}_{b}$$\end{document} Q b : buffer flow rate, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${Q}_{sc}$$\end{document} Q sc : suction flow rate, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${w}_{p}$$\end{document} w p : width of pinched segment, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${w}_{b}$$\end{document} w b : width of broadened segment

Article Snippet: Biologically intact Exosome Separation Technology, BEST , - High yield & high purity - Single step -continuous Conseparation - Handle from 100 uL of sample - Direct processing of cell culture, blood, urine, etc. - Damage-free and contamination-free intact EVs , - Need throughput increase.

Techniques: Field Flow Fractionation, Diffusion-based Assay, Fractionation, Shear